rabbit polyclonal antipgc1α antibody Search Results


rabbit polyclonal antipgc1α antibody  (Novus Biologicals)
93
Novus Biologicals rabbit polyclonal antipgc1α antibody
Rabbit Polyclonal Antipgc1α Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1+alpha+Antibody+%5BPerCP%5D/pm37749090-331-28-36
Average 93 stars, based on 1 article reviews
rabbit polyclonal antipgc1α antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
rabbit anti pgc1α antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti pgc1α antibody
Rabbit Anti Pgc1α Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC-1alpha+Rabbit+mAb/pm41075110-116-5-10
Average 96 stars, based on 1 article reviews
rabbit anti pgc1α antibody - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
rabbit anti pgc1α  (Proteintech)
96
Proteintech rabbit anti pgc1α
Rabbit Anti Pgc1α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1a+Antibody/bio_rxiv__194654-63-58-60
Average 96 stars, based on 1 article reviews
rabbit anti pgc1α - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti pgc1α  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti pgc1α
Anti Pgc1α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC-1%CE%B1+Antibody/pmc05701255-226-28-33
Average 96 stars, based on 1 article reviews
anti pgc1α - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
rabbit polyclonal anti pgc1α antibody  (Danaher Inc)
99
Danaher Inc rabbit polyclonal anti pgc1α antibody
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Rabbit Polyclonal Anti Pgc1α Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc08673768-394-106-113
Average 99 stars, based on 1 article reviews
rabbit polyclonal anti pgc1α antibody - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
rabbit polyclonal antipgc 1α  (Novus Biologicals)
96
Novus Biologicals rabbit polyclonal antipgc 1α
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Rabbit Polyclonal Antipgc 1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1+alpha+Antibody+-+BSA+Free/pm38216792-118-57-60
Average 96 stars, based on 1 article reviews
rabbit polyclonal antipgc 1α - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti pgc1α  (OriGene)
90
OriGene anti pgc1α
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Anti Pgc1α, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1+alpha+(PPARGC1A)+Rabbit+Polyclonal+Antibody/us09771589-209-12-13
Average 90 stars, based on 1 article reviews
anti pgc1α - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit anti pgc1α  (Novus Biologicals)
93
Novus Biologicals rabbit anti pgc1α
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Rabbit Anti Pgc1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1+alpha+Antibody/pmc04167222-86-26-28
Average 93 stars, based on 1 article reviews
rabbit anti pgc1α - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
anti pgc1α  (Boster Bio)
94
Boster Bio anti pgc1α
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Anti Pgc1α, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/Anti-PGC1+Alpha+%2F+Beta+PPARGC1B+Antibody/pmc10572372-59-9-10
Average 94 stars, based on 1 article reviews
anti pgc1α - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
rabbit anti pgc1α antibody chip grade  (Novus Biologicals)
96
Novus Biologicals rabbit anti pgc1α antibody chip grade
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Rabbit Anti Pgc1α Antibody Chip Grade, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/PGC1+alpha+Antibody+-+BSA+Free/pmc07197710-216-123-132
Average 96 stars, based on 1 article reviews
rabbit anti pgc1α antibody chip grade - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
rabbit anti pgc1α  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti pgc1α
( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) <t>PGC1α</t> and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.
Rabbit Anti Pgc1α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antipgc1%CE%B1+antibody/Phospho-p53+(Ser15)+Antibody/pm40344568-132-23-26
Average 96 stars, based on 1 article reviews
rabbit anti pgc1α - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) PGC1α and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.

Journal: Science Advances

Article Title: Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance

doi: 10.1126/sciadv.abl4988

Figure Lengend Snippet: ( A to B ) Gastrocnemius muscles from 3- or 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were processed for qPCR monitoring for the expression of mitochondrial biogenesis genes. ( C to F ) Gastrocnemius muscle from 6-month-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice was homogenized and immunoblotted for (C) PGC1α and (D) total OXPHOS proteins [complexes (C) I to V] or processed for the analysis of (E) citrate synthase (CS) activity. (F) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were frozen, and transverse sections were processed for SDH staining and Tomm20 along with dystrophin immunostaining to define mitochondria within individual muscle fibers. ( G ) Gastrocnemius muscle from 6-month-old male Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed (4.8% fat) mice was homogenized. Proteins were digested with trypsin and analyzed on a QExactive HF mass spectrometer. KEGG and differentially regulated proteins were identified considering a P value and log 2 fold-change cutoff of ≤0.05 and >|0.3|, respectively. A heatmap of selected differentially expressed proteins associated with OXPHOS is shown. ( H and I ) Gastrocnemius muscles from 6-month-old male Nox4 fl/fl , Mck -Cre; Nox4 fl/fl , and Mck -Cre; Nox4 fl/fl ; Gpx1 −/− chow-fed (4.8% fat) mice were processed for (I) qPCR or ( J ) immunoblotting. (J and K ) Twelve-week-old Nox4 fl/fl and Mck -Cre; Nox4 fl/fl chow-fed male mice were subjected to an acute bout of exercise on multilane treadmill for 50 min at moderate intensity (70% VO 2 max). After 4 hours, gastrocnemius muscles were excised from sedentary (Sed) and exercised (Ex) mice and processed for (J) qPCR or (K) immunoblotting. Representative and quantified results are shown (means ± SEM) for the indicated number of mice. Significance determined using (A to C and E) a Student’s t test, (H and I) a one-way ANOVA, or (J and K) a two-way ANOVA; # indicates significance using a Student’s t test.

Article Snippet: The primary antibodies used for immunoblotting include rabbit monoclonal anti–β-actin (A5441, RRID:AB_476744), mouse monoclonal anti–α-tubulin (T5168, RRID:AB_477579), mouse monoclonal anti-vinculin (V9131, RRID:AB_477629), and rabbit monoclonal anti-NFE2L2 (SAB2701989) from Sigma-Aldrich (St. Louis, MO); rabbit monoclonal anti–phospho-AKT (p-AKT) (473) (4060, RRID:AB_2315049), mouse monoclonal anti-AKT (40D4, RRID:AB_1147620), and rabbit polyclonal anti-PRDX1 (8732) from Cell Signaling Technology (Danvers, MA); rabbit polyclonal anti-catalase (ab1877, RRID:AB_302649), rabbit polyclonal anti-G6PD (ab993, RRID:AB_296714), rabbit anti–4-HNE (ab46545, RRID:AB_722490), rabbit monoclonal anti-KEAP1 (ab139729, RRID:AB_2891077), mouse monoclonal anti-myogenin (ab1835, RRID:AB_302633), goat polyclonal anti-NCF1/p47 phox (ab166930, RRID:AB_2832946), mouse monoclonal anti-PRDX2 [EPR5154] (ab109367, RRID:AB_10862524), rabbit polyclonal anti-PRDX3 (ab222807), rodent anti-OXPHOS cocktail (ab110413, RRID:AB_2629281), rabbit monoclonal anti-NFE2L2 (ab62352, RRID:AB_944418), and rabbit polyclonal anti-PGC1α antibody (ab54481, RRID:AB_881987) from Abcam (Cambridge, UK); rabbit polyclonal anti-NOX4 (sc-30141, RRID:AB_2151703), rabbit polyclonal anti-IRβ (sc-711, RRID:AB_631835), and mouse monoclonal anti-SOD2 (E-10, RRID:AB_2191808) from Santa Cruz Biotechnology (Santa Cruz, CA); rabbit monoclonal anti–IRS-1 (06-248, RRID:AB_2127890) and rabbit monoclonal anti-PI3K p85 (06-497, RRID:AB_310141) from Upstate Biotechnology (Lake Placid, NY); mouse monoclonal anti-gp91 phox (611415, RRID:AB_398937) and mouse monoclonal anti-RAC1 (610651, RRID:AB_397978) from BD Biosciences (San Jose, CA); mouse monoclonal anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (AM4300, RRID:AB_2536381) from Thermo Fisher Scientific (Waltham, MA); mouse monoclonal anti-NQO1 (NB200-209, RRID:AB_10002706) from Novus Biologicals (Littleton, CO); and mouse monoclonal anti-MHC (MYH1E) (MF20) from J. M. Ziermann Laboratory (Howard University College of Medicine, Developmental Studies Hybridoma Bank, University of Iowa, USA).

Techniques: Muscles, Expressing, Activity Assay, Staining, Immunostaining, Mass Spectrometry, Western Blot

( A to I ) Skeletal muscle myoblasts were FACS-purified from Nox4 fl/fl mice and transduced with β-galactosidase (LacZ) control or Cre recombinase–expressing adenoviruses to delete Nox4. The resultant cells were differentiated into myotubes and processed for (A) qPCR or (B) immunoblotting to assess NOX4 levels, (C) qPCR to monitor mitochondrial biogenesis gene expression, (D) immunoblotting to monitor for PGC1α, or (E) OXPHOS protein levels [complexes (C) I to V; protein levels were quantified by densitometry]. (F) Mitochondrial respiration was assessed in live myotubes by performing the Seahorse XF Cell Mito Stress Test and measuring the oxygen consumption rate (OCR); basal respiration and maximal respiration, after inhibiting ATP synthase with oligomycin and uncoupling respiration with FCCP, were assessed. Myotubes were processed for (G) qPCR and (H) immunoblotting to assess NFE2L2 levels or (I) qPCR to assess antioxidant defense gene expression. Representative and quantified results are shown (means ± SEM) for the indicated number of experiments; significance determined using (A, C, D, and F to I) a Student’s t test or (E) two-way ANOVA.

Journal: Science Advances

Article Title: Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance

doi: 10.1126/sciadv.abl4988

Figure Lengend Snippet: ( A to I ) Skeletal muscle myoblasts were FACS-purified from Nox4 fl/fl mice and transduced with β-galactosidase (LacZ) control or Cre recombinase–expressing adenoviruses to delete Nox4. The resultant cells were differentiated into myotubes and processed for (A) qPCR or (B) immunoblotting to assess NOX4 levels, (C) qPCR to monitor mitochondrial biogenesis gene expression, (D) immunoblotting to monitor for PGC1α, or (E) OXPHOS protein levels [complexes (C) I to V; protein levels were quantified by densitometry]. (F) Mitochondrial respiration was assessed in live myotubes by performing the Seahorse XF Cell Mito Stress Test and measuring the oxygen consumption rate (OCR); basal respiration and maximal respiration, after inhibiting ATP synthase with oligomycin and uncoupling respiration with FCCP, were assessed. Myotubes were processed for (G) qPCR and (H) immunoblotting to assess NFE2L2 levels or (I) qPCR to assess antioxidant defense gene expression. Representative and quantified results are shown (means ± SEM) for the indicated number of experiments; significance determined using (A, C, D, and F to I) a Student’s t test or (E) two-way ANOVA.

Article Snippet: The primary antibodies used for immunoblotting include rabbit monoclonal anti–β-actin (A5441, RRID:AB_476744), mouse monoclonal anti–α-tubulin (T5168, RRID:AB_477579), mouse monoclonal anti-vinculin (V9131, RRID:AB_477629), and rabbit monoclonal anti-NFE2L2 (SAB2701989) from Sigma-Aldrich (St. Louis, MO); rabbit monoclonal anti–phospho-AKT (p-AKT) (473) (4060, RRID:AB_2315049), mouse monoclonal anti-AKT (40D4, RRID:AB_1147620), and rabbit polyclonal anti-PRDX1 (8732) from Cell Signaling Technology (Danvers, MA); rabbit polyclonal anti-catalase (ab1877, RRID:AB_302649), rabbit polyclonal anti-G6PD (ab993, RRID:AB_296714), rabbit anti–4-HNE (ab46545, RRID:AB_722490), rabbit monoclonal anti-KEAP1 (ab139729, RRID:AB_2891077), mouse monoclonal anti-myogenin (ab1835, RRID:AB_302633), goat polyclonal anti-NCF1/p47 phox (ab166930, RRID:AB_2832946), mouse monoclonal anti-PRDX2 [EPR5154] (ab109367, RRID:AB_10862524), rabbit polyclonal anti-PRDX3 (ab222807), rodent anti-OXPHOS cocktail (ab110413, RRID:AB_2629281), rabbit monoclonal anti-NFE2L2 (ab62352, RRID:AB_944418), and rabbit polyclonal anti-PGC1α antibody (ab54481, RRID:AB_881987) from Abcam (Cambridge, UK); rabbit polyclonal anti-NOX4 (sc-30141, RRID:AB_2151703), rabbit polyclonal anti-IRβ (sc-711, RRID:AB_631835), and mouse monoclonal anti-SOD2 (E-10, RRID:AB_2191808) from Santa Cruz Biotechnology (Santa Cruz, CA); rabbit monoclonal anti–IRS-1 (06-248, RRID:AB_2127890) and rabbit monoclonal anti-PI3K p85 (06-497, RRID:AB_310141) from Upstate Biotechnology (Lake Placid, NY); mouse monoclonal anti-gp91 phox (611415, RRID:AB_398937) and mouse monoclonal anti-RAC1 (610651, RRID:AB_397978) from BD Biosciences (San Jose, CA); mouse monoclonal anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (AM4300, RRID:AB_2536381) from Thermo Fisher Scientific (Waltham, MA); mouse monoclonal anti-NQO1 (NB200-209, RRID:AB_10002706) from Novus Biologicals (Littleton, CO); and mouse monoclonal anti-MHC (MYH1E) (MF20) from J. M. Ziermann Laboratory (Howard University College of Medicine, Developmental Studies Hybridoma Bank, University of Iowa, USA).

Techniques: Purification, Transduction, Control, Expressing, Western Blot, Gene Expression